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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been observed in many tumors, which ends up in chromosome instability, and therefore may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up claimed but in depth nucleotide sequence data will not be nonetheless out there.

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Blocking peptides are peptides that bind particularly to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...

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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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